The College of Pharmacy provides a variety of equipment and services supporting all research groups.
We have in-house instrumentation, computing, electronics and facilities specialists, as well as a wide spectrum of resources deployed throughout the college.
The college maintains shared resources available to all college research personnel. College research personnel also have access to the instrumentation and services (NMR, MS and proteomics) of the Ohio State Campus Chemical Instrument Center, including the 600 and 800MHz NMR spectrometers located adjacent to the college’s 400MHz instrument.
Please consult the list below which provides a short description of the equipment, its location, and links to user manuals and scheduling through http://fom.osu.edu. Please also consult the list of software available within the college. For questions, comments, or training please contact the Director of Instrumentation, Craig McElroy, at email@example.com or 614-292-3232.
Instruments in service
The Agilent 1100 series analytical HPLC produces flow rates from 0.001-5 ml/min, handles back pressures up to 400 bar, and allows automated injection of samples from vials or plates. The column compartment allows for column temperatures ranging from 10° below ambient to 80°C and contains a switching valve to automatically switch between two columns. The diode array detector allows for acquisition of full spectrum data with a 20 Hz data sampling rate. The fluorescence detector allows for monitoring of up to 4 signals with excitation ranging from 200-700 nm and emission ranging from 280-900 nm.
The Agilent 1200 series purification system is designed to handle flow rates up to 100 ml/min for laboratory-scale purification of milligram-gram quantities of material. The flow rate range is ideal for columns with internal diameters from 9.4-50 mm. The dual piston preparative pumps handle back pressures up to 400 bar. The dual-loop autosampler allows automated injection of samples from 0.1 µl-10 ml from either vials or well-plates. The diode array detector allows for acquisition of full spectrum data (190-950 nm) with a 20 Hz data sampling rate. The fraction collector with patented fraction delay sensor allows for fraction triggers based on time and/or peak sensing.
Available columns for the HPLC:
- Beckman ultrasphere ODS C18 – 250x4.6mm column with 5 µm particle size and 80 Å pore size
- Phenomenex Ultremex C18 – 250x10mm column with 5 µm particle size and 80 Å pore size
- Phenomenex Gemini C18 – 250x21.2mm column with 5 µm particle size and 110 Å pore size
- Varian Microsorb C18 – 250x41.4mm column with 8 µm particle size and 100 Å pore size
The ÄKTA fast protein liquid chromatography system allows for flow rates from 0.05-20 ml/min with a maximum operating pressure of 5 MPa. The single wavelength detector allows for detection at either 254 or 280 nm with a 5 mm flow cell path. The Frac-950 automated fraction collector allows for collection of up to one hundred twenty 18 mm tubes and eight 30 mm tubes at one time. The system is also equipped with a superloop that allows for injection of sample volumes up to 50 ml.
The Nucleofector™ Technology is based on two unique components, the Nucleofector™ Device that delivers the specifically developed electrical parameters and Nucleofector™ Kits that contain cell-specific and optimized Nucleofector™ Solutions. With over 100 protocols for cell lines optimized by Lonza’s R&D team, more than 500 entries in the online cell data base and almost 50 Nucleofector™ Kits for different primary cells, the Nucleofector™ Technology is clearly the transfection method-of-choice for virtually any difficult-to-transfect cell type.
The Anton Paar MCP 150 polarimeter measures optical rotation with an accuracy of 0.005 ° with an automatic Peltier temperature control from 15 °C to 35 °C, three default measuring modes (optical rotation, specific rotation, and concentration), and a 0.7 ml internal volume Hastelloy C-276 cell with excellent solvent compatibility.
The ProFlex PCR System is an end-point thermal cycler with a touchscreen graphical display that shows the time, status, and temperature for each run allowing the user to enter information into fields on the display screen. The instrument has interchangeable blocks, and detects which module is present on the system automatically. Available blocks include the: 3x32-Well Sample Block, 96-Well Block, and Dual Flat Sample Block.
The QuantStudio 7 Flex features a simplified workflow, intuitive software, automation capabilities, and a touch-screen interface with exceptional reproducibility and minimal well-to-well and instrument-to-instrument variation. It enables a broad range of real-time PCR-based applications through its multiplexing capabilities and interchangeable block formats. Available blocks include the: 384-well Block and Array Card Block. Additionally the Array Card Stacker/Sealer is available for setting up the Array Cards.
Innovative VeriFlex Blocks allow for precise PCR optimization and enable you to run up to 6 independent assays using standard 0.2 mL block format with standard and fast enabled cycling to address your current and future PCR needs, easy-to-use graphical interface through a 6.5 inch VGA Touch Screen, convenient protocol transfer from one Veriti® Thermal Cycler to another using USB and reduced PCR cycling time when using faster ramp rates.
High speed preparatory centrifuge with JLA-16.250, JA-18, and JA-25.50 rotors that includes high-torque switched reluctance drive for fast separations, two acceleration and three deceleration rates, and a maintenance-free partial vacuum system that reduces friction on the rotor.
High speed preparatory ultracentrifuge with SW 28, SW 41, 50.2 TI, and 70 TI rotors that includes a vacuum-encased induction drive, microprocessor-based control, environmentally-friendly cooling system, and high performance drive technology with speeds up to 100,000 rpm
Liquid scintillation counter with 32,768 channel analyzer yielding an effective resolution of 0.06 keV per channel, patented H-Number Plus external quench monitor yielding accurate disintegrations per minute (DPM) and automated quench compensation independent of sample volume or cocktail composition and allowing color detection and correction, two-phase monitoring, unquenched window determination, and multi-label DPM quench curves.
Flow cytometer with automated tube-based acquisition for clinical assays. It has the capacity to conduct 5-color analysis using the single 488 nm (blue) laser and has a carousel tube loader for automated sample loading.
Particle counter capable of absolute cell counts or concentration, single-button calibration, using a technology defined by the International Standard (ISO 13319:2000), stores up-to five analyses settings, allows operator-selectable size settings, and is a dual-threshold model for working with platelets or numerous cell types of variable sizes. Count cells or particles in three regions: equal to-or-above the operator-selected lower size, above the upper size-setting, and between the two size selections.
PCR thermocycler with interchangeable reaction modules (only have the 96x0.2ml module) 3.3 °C/sec heating rate, 2 °C/sec cooling rate, Peltier and joule heating and cooling, temperature range from 4-100 °C, accuracy of ±0.3 °C, uniformity of ±0.4 °C, overshoot maximum of <0.5 °C, and heated lid with temperatures up to 105 °C
Real-Time PCR thermocycler capable of two-target analysis with an average ramp rate of 3.3 °C/sec using a Peltier heating and cooling system, with a temperature range from 0-100 °C, an accuracy of ±0.2 °C, uniformity of ±0.4 °C, 3 filtered LEDs for excitation and 3 filtered photodiodes for detection, excitation/emission range of 450-580 nm, with single copy sensitivity for genomic DNA, and 10 orders of magnitude dynamic range with a sample size of 1-50 µl.
A positive pressure manifold designed to process 96-well extraction plates (SPE, SLE, filtration, etc.) using a pressurized gas source for bioanalytical sample processing. The instrument accepts most 96-well plate designs, including fixed-well plates and modular arrays.
Provides microplate priming and washing for ELISA, fluorescence and chemiluminescence immunoassays, cellular and agglutination assays. Special plate carriers allow for vacuum filtration with three vacuum levels and biomagnetic separation magnet to retain magnetic beads during aspiration. Programmable dispense volumes, flow, and height, and aspiration rates and depth.
A hybrid multi-mode microplate reader capable of fluorescence intensity (FI), fluorescence polarization (FP), time resolved fluorescence (TRF), luminescence, and UV-visible absorbance readings and dual-reagent dispensing to 6-, 12-, 24-, 48-, 96-, and 384-well microplates. Take3 micro-volume plate allows for readings with just 2 µl of sample.
The Bruker Avance NEO features faster control, improved dynamic range and greater flexibility and scalability. Each NMR channel in the Avance NEO has both transmit and receive capabilities making each an independent spectrometer with the full RF generation, transmission and receive infrastructure allowing for multi-receiver experiments to be run. Also new in the AVANCE NEO is the concept of an embedded acquisition server and an associated client-server software architecture (TopSpin 4 and higher) leaving the spectrometer independent of the client computer.
Computer with dual screen monitors and all of the appropriate software installed to analyze and process all data collected from all of the instruments within the instrumentation center. ACDLabs Percepta and ACDLabs Spectrus software are also installed.
The EVOS® FL Imaging System is a fully integrated, digital, inverted imaging system for four-color fluorescence and transmitted-light applications. It is powerful, yet easy-to-use and delivers high-definition images with exceptional convenience. The unique light cubes, sensitive camera, and precision-engineered optical system make the EVOS® FL system ideal for both demanding and routine fluorescence imaging applications.
The EVOS XL Core Imaging System is a digital transmitted-light inverted imaging system for cell and tissue culture applications and routine cell maintenance. Its simple user interface drives a color camera and high-quality optical system that delivers high-definition images with exceptional ease. Equipped with 4X, 10X, 20X, and 40X long-working–distance (LWD) phase contrast (PH) objectives and a mechanical stage, the all-in-one EVOS XL Core system is an ideal microscope for basic imaging needs.
Water jacketed CO2 incubator capable of maintaining temperature from 5 °C above ambient temperature to 60 °C with an accuracy of ±0.2 °C and gas system controls capable of maintaining the setpoint to within ±0.1%.
Spectropolarimeter equipped for circular dichroism (CD), optical rotary dispersion (ORD) and linear dichroism (LD) detection with four channel simultaneous data acquisition, three scanning modes (continuous scan/step scan/auto response scan), wavelength range down to 163 nm, baseline stability <0.03 m°/hr, noise level at 200 nm 0.040 m° and at 185 nm 0.05 m°, spectral bandwidth of 0.01 to 15 nm, slit width of 1 to 3000 µm, response of 0.5 msec to 32 sec, auto scale during data acquisition, and PC control of shutter, nitrogen purge control, PMT HT settings, and light source power
Labconco model 7810010 Centrivap centrifugal concentrator with model 7811020 cold trap (-55 °C), model 7455100 rotor (for 12-13 mm tubes), and model 7914100 corrosion resistant diaphragm pump (42 liters/min, 23.8 in. Hg vacuum). For use in evaporating aqueous samples in tubes or plates.
GC/MS with isolated ion source offering fast source swapping and automatic source identification, a linear dynamic range of 4 or more orders of magnitude, up to 80 full-range mass spectra/second, and both Electron ionization (EI) and chemical ionization (CI) sources.
Water treatment system that produces 2-100 L of type 1 ultrapure water (18.2 MΩcm) per day with a flow rate of 1.5 L/min yielding water quality suitable for HPLC, GC, ILC, and TPC analyses.
With ultraviolet, visible, fluorescence, time-resolved fluoresence, fluorescence polarization, and luminescence capabilities with top and bottom read equipped with a StackMax microplate handling system providing automated reading of up to 50 microplates.
48 liter capacity CO¬2 incubator with LCD interface, 72-hour data logging, easy access setpoints and alarms, O2 control, high-temperature disinfection, and outer door viewing window.
Nikon Eclipse Ti microscope with 4X, 10X, 20X, and 60X oil immersion objectives with Xcite 120 LED light source with speed dial and CoolSNAP ES2 camera. Capable of DAPI, BFP, CFP, GFP, and TexRed imaging using NIS-Elements BR 3.22.11 software.
Class II A2 laminar airflow biological safety cabinet with a 58 3/8” x 23 1/2" work space.
Olympus BX61TRF spinning disk confocal microscope with a Hamamatsu Orca Flash 4.0LT EM-CCD camera, Olympus SC30 color camera, an Olympus BX-UCB motorized controller unit, an Olympus U-LH100HGAPO reflected fluorescence system, an Olympus BH2-RFL-T3 high pressure mercury burner and reflected light fluorescence attachment, and 4X, 10X, 40X, and 60X (water and oil immersion) objectives.
Digital darkroom imager with a 5-log dynamic range large/high-resolution CCD sensor, RGB fluorescent and epi-excitation (632 nm red, 534 nm green and 475 nm blue LEDs, 302 nm UV transilluminator, and epi-white lights and trans-white light), 10-position motorized filter wheel for emission (710/40 nm filter, 607/36 nm filter, 593/40 nm filter, 537/26 nm filter), and integrated computer with LCD touch screen.
Benchtop assay platform equipped with state-of-the-art optics, full autofocus, and image analysis software for rapid assessment of cells in suspension. With three-channel flexibility—brightfield and two optional fluorescence channels— to count cells, monitor fluorescent protein expression, evaluate apoptosis, and measure cell viability.
LTQ Orbitrap is a hybrid mass spectrometer incorporating the LTQ linear trap for sample ionization, selection, fragmentation, and automatic gain control, a curved linear trap for intermediate storage and short pulse injection; an Orbitrap™ analyzer for Fourier transformation based analysis; and a collision cell for performing CID experiments. The LTQ Orbitrap is capable of a resolution of 60 000 (FWHM) @ m/z 400 with a scan repetition rate of 1 second with a minimum resolution of 7500 and a maximum resolution of 100000 @ m/z 400, a mass range of m/z 50–2000 (LTQ) and m/z 200–4000 (Orbitrap), a mass accuracy of <5 ppm RMS for 3 days with external calibration or <2 ppm RMS with internal calibration, a dynamic Range of >10000 between mass spectra and >4 000 between highest and lowest detectable mass in one spectrum, capable of MS/MS and MSn scan functions.
TSQ quantiva is a triple quadrupole mass spectrometer with a scan rate of 15,000 amu/second at a resolution of 2 FWHM that can collect 500 SRMs/second (for any resolution from 0.2 through 2.0 FWHM), a resolution of 0.2 Da peak width (FWHM) across the entire mass range, a mass range of m/z 10-1850, and a mass stability of ±0.05 Da over a 24 hour period. It is connected to a Thermo Vanquish UHPLC system (see below)
The Vanquish UHPLC system allows for pressures up to 1500 bar (22,000 psi) at flow rates up to 5 mL/min with an industry-leading flow and gradient precision and 2 x 3 solvent channels. The autosampler provide excellent injections up to 100 µL in 0.01 µL increments with incredible injection linearity even below 1 µl while allowing for automation of workflows with barcode reading. The column oven allows for 2 thermostatting modes (passive and forced air) with a 5 ºC to 120 ºC temperature range and provides active pre-heating. All connections in the system are made with Viper capillaries with zero dead volume and finger tight installation.
Capillary melting point apparatus with a magnifying lens and an electrically heated silicone oil bath with backlighting, a stirrer to eliminate thermal gradients, a thermometer guard with an adjustable periscope and hairline for accurate thermometer readings, and backlighting for the thermometer.
The Quattro Premier is a triple quadrupole mass spectrometer configured for Integrated HPLC-API-MS-MS, providing molecular weight information, structural information and quantitative analysis for a wide range of compounds. It is connected to a Waters Acquity UPLC system (see below).
ACQUITY UPLC System outperforms traditional or optimized HPLC by allowing chromatographers to work at higher efficiencies with a much wider range of linear velocities, flow rates, and backpressures. (Scheduled in conjunction with the Quattro Premier above)
Software for NMR data processing and analysis. Two computers with the Topspin software are set up for use in Parks 335A. Additionally, Topspin can be installed on College owned computers by e-mailing firstname.lastname@example.org.
Software for structure confirmation and output of NMR data in journal specified formats. Two computers with the CMCassist software are set up for use in Parks 335A. Additionally, CMCassist can be installed on College owned computers by e-mailing email@example.com.
Software for automated/semi-automated structure elucidation of unknowns. Two computers with the CMCse software are set up for use in Parks 335A. Additionally, CMCse can be installed on College owned computers by e-mailing firstname.lastname@example.org.
Molecular visualization software for viewing/manipulating 3D structures of small and macromolecules. Pymol can be installed on College owned computers by e-mailing email@example.com.
Software for in silico prediction of physicochemical; absorption, distribution, metabolism, excretion (ADME); and toxicity properties to help understand structure-property relationships, and explain the behaviour of novel compounds in the life sciences. A computer with the percepta software is set up for use in Parks 335A.
Software for data processing and database generation for data from the majority of instruments within the Instrumentation facility.
Software for chemical structure drawing and analysis combined with biological pathway drawing. ChemBioDraw Ultra can be installed on College owned computers by registering for a code using your OSU email address at http://go.osu.edu/chemdraw and then send the codes to firstname.lastname@example.org to have the software installed.