Dan Shu

Image
Dan Shu

Dan Shu, PhD

Title
Research Associate Professor
Unit / Department / Division
Pharmaceutics and Pharmacology
Address
914 Biomedical Research Tower Biomedical Research Tower
Email Address
Phone Number

Professional Interests

Professional Interests

Dr. Shu’s research is focused on (1) RNA nanotechnology: use RNA as a nanotechnology platform to construct RNA nanoparticles with functional modalities for therapeutics, targeting, and diagnostics (ACS Nano, 2015; Nature Nanotechnology, 2011; Nano Letters, 2004); and (2) Single molecule biophysical studies: use single-molecule detection (such as FRET) to understand the structure-function relationship for individual biomolecules (ACS Nano, 2010; EMBO Journal, 2007; RNA, 2007). Her long-term objective is to focus on the development and translation of RNA nanotechnology-based therapeutics and imaging agents into clinical trials.

Education

  • 1990, M.D., Tongji Medical University

Journal Articles

  • Xu, Y.; Pang, L.; Wang, H.; Xu, C. et al. Specific delivery of delta-5-desaturase siRNA via RNA nanoparticles supplemented with dihomo-γ-linolenic acid for colon cancer suppression Redox Biol. 2018 Dec; 21 101085-101085.
  • Yin, H.; Wang, H.; Li, Z.; Shu, D. et al. RNA Micelles for Systemic Delivery of Anti-miRNA for Cancer Targeting and Inhibition without Ligand ACS Nano. 2018 Dec; .
  • Shu, Y.; Yin, H.; Rajabi, M.; Li, H. et al. RNA-based micelles: A novel platform for paclitaxel loading and delivery J Control Release. 2018 Apr; 276 17-29.
  • Smith, J.A.; Braga, A.; Verheyen, J.; Basilico, S. et al. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity Mol Ther Nucleic Acids. 2018 Mar; 10 103-121.
  • Xu, C.; Haque, F.; Jasinski, D.L.; Binzel, D.W. et al. Favorable biodistribution, specific targeting and conditional endosomal escape of RNA nanoparticles in cancer therapy Cancer Lett. 2018 Feb; 414 57-70.
  • Yang, X.; Xu, Y.; Wang, T.; Shu, D. et al. Erratum to "Inhibition of cancer migration and invasion by knocking down delta-5-desaturase in COX-2 overexpressed cancer cells" [Redox Biol. 11 (2017) 653-662] Redox Biol. 2017 Aug; 12 1062-1062.
  • Zhao, Z.; Zhang, H.; Shu, D.; Montemagno, C. et al. Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination Small. 2017 Jan; 13(1):.
  • Li, H.; Zhang, K.; Pi, F.; Guo, S. et al. Controllable Self-Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting Adv Mater. 2016 Sep; 28(34):7501-7507.
  • Binzel, D.W.; Shu, Y.; Li, H.; Sun, M. et al. Specific Delivery of MiRNA for High Efficient Inhibition of Prostate Cancer by RNA Nanotechnology Mol Ther. 2016 Aug; 24(7):1267-1277.
  • Li, H.; Rychahou, P.G.; Cui, Z.; Pi, F. et al. RNA Nanoparticles Derived from Three-Way Junction of Phi29 Motor pRNA Are Resistant to I-125 and Cs-131 Radiation Nucleic Acid Ther. 2015 Aug; 25(4):188-197.
  • Cui, D.; Zhang, C.; Liu, B.; Shu, Y. et al. Regression of Gastric Cancer by Systemic Injection of RNA Nanoparticles Carrying both Ligand and siRNA Sci Rep. 2015 Jul; 5 10726-10726.
  • Shu, D.; Pi, F.; Wang, C.; Zhang, P. et al. New approach to develop ultra-high inhibitory drug using the power function of the stoichiometry of the targeted nanomachine or biocomplex Nanomedicine (Lond). 2015 Jul; 10(12):1881-1897.
  • Lee, T.J.; Haque, F.; Shu, D.; Yoo, J. et al. RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model Oncotarget. 2015 Jun; 6(17):14766-14776.
  • Lee, T.J.; Haque, F.; Shu, D.; Yoo, J.Y. et al. RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model. Oncotarget. 2015 Jun; 6(17):14766-14776.
  • Feng, L.; Li, S.K.; Liu, H.; Liu, C-Y. et al. Ocular delivery of pRNA nanoparticles: distribution and clearance after subconjunctival injection Pharm Res. 2014 Apr; 31(4):1046-1058.
  • Shu, Y.; Pi, F.; Sharma, A.; Rajabi, M. et al. Stable RNA nanoparticles as potential new generation drugs for cancer therapy Adv Drug Deliv Rev. 2014 Feb; 66 74-89.
  • Shu, D.; Khisamutdinov, E.F.; Zhang, L.; Guo, P. Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor Nucleic Acids Res. 2014 Jan; 42(2):e10-e10.
  • Shu, Y.; Shu, D.; Haque, F.; Guo, P. Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells Nat Protoc. 2013 Sep; 8(9):1635-1659.
  • Shu, Y.; Haque, F.; Shu, D.; Li, W. et al. Fabrication of 14 different RNA nanoparticles for specific tumor targeting without accumulation in normal organs RNA. 2013 Jun; 19(6):767-777.
  • Haque, F.; Shu, D.; Shu, Y.; Shlyakhtenko, L.S. et al. Ultrastable synergistic tetravalent RNA nanoparticles for targeting to cancers Nano Today. 2012 Aug; 7(4):245-257.
  • Shu, D.; Shu, Y.; Haque, F.; Abdelmawla, S. et al. Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics Nat Nanotechnol. 2011 Sep; 6(10):658-667.
  • Shu, Y.; Cinier, M.; Shu, D.; Guo, P. Assembly of multifunctional phi29 pRNA nanoparticles for specific delivery of siRNA and other therapeutics to targeted cells Methods. 2011 Jun; 54(2):204-214.
  • Jing, P.; Harque, F.; Shu, D.; Montemagno, C. et al. Unidirectional Translocation of DNA Through the Phi29 Connector Channel Biophysical Journal. 2011 Feb; 100(3):167-167.
  • Shu, D.; Zhang, H.; Petrenko, R.; Meller, J. et al. Dual-channel single-molecule fluorescence resonance energy transfer to establish distance parameters for RNA nanoparticles ACS Nano. 2010 Nov; 4(11):6843-6853.
  • Jing, P.; Haque, F.; Shu, D.; Montemagno, C. et al. One-way traffic of a viral motor channel for double-stranded DNA translocation Nano Lett. 2010 Sep; 10(9):3620-3627.
  • Zhang, H.; Shu, D.; Browne, M.; Guo, P. Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motor Biomed Microdevices. 2010 Feb; 12(1):97-106.
  • Zhang, H.; Shu, D.; Petrenko, R.; Lee, T. et al. Dual-Channel Single-Molecule Imaging of pRNA on phi29 DNA-Packaging Motor Biophysical Journal. 2010 Jan; 98(3):579A-579A.
  • Zhang, H.; Shu, D.; Wang, W.; Guo, P. Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF Proc SPIE Int Soc Opt Eng. 2010 Jan; 7571 757107-757108.
  • Li, L.; Liu, J.; Diao, Z.; Shu, D. et al. Evaluation of specific delivery of chimeric phi29 pRNA/siRNA nanoparticles to multiple tumor cells Mol Biosyst. 2009 Nov; 5(11):1361-1368.
  • Shu, Y.; Shu, D.; Diao, Z.; Shen, G. et al. Fabrication of Polyvalent Therapeutic RNA Nanoparticles for Specific Delivery of siRNA, Ribozyme and Drugs to Targeted Cells for Cancer Therapy IEEE NIH Life Sci Syst Appl Workshop. 2009 May; 2009 9-12.
  • Zhang, H.; Shu, D.; Browne, M.; Guo, P. Approaches for stoichiometry and distance determination of nanometer bio-complex by dual-channel single molecule imaging IEEE NIH Life Sci Syst Appl Workshop. 2009 Apr; 2009 124-127.
  • Ko, S.H.; Chen, Y.; Shu, D.; Guo, P. et al. Reversible switching of pRNA activity on the DNA packaging motor of bacteriophage phi29 J Am Chem Soc. 2008 Dec; 130(52):17684-17687.
  • Chang, C-L.; Zhang, H.; Shu, D.; Guo, P. et al. Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system Appl Phys Lett. 2008 Oct; 93(15):153902-153902.
  • Fang, Y.; Shu, D.; Xiao, F.; Guo, P. et al. Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging Biochem Biophys Res Commun. 2008 Aug; 372(4):589-594.
  • Zhang, H.; Shu, D.; Huang, F.; Guo, P. Instrumentation and metrology for single RNA counting in biological complexes or nanoparticles by a single-molecule dual-view system RNA. 2007 Oct; 13(10):1793-1802.
  • Shu, D.; Zhang, H.; Jin, J.; Guo, P. Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system EMBO J. 2007 Jan; 26(2):527-537.
  • Shu, D.; Moll, W-D.; Deng, Z.; Mao, C. et al. Bottom-up Assembly of RNA Arrays and Superstructures as Potential Parts in Nanotechnology Nano Lett. 2004 Sep; 4(9):1717-1723.
  • Shu, D.; Huang, L.; Guo, P. A simple mathematical formula for stoichiometry quantification of viral and nanobiological assemblage using slopes of log/log plot curves J Virol Methods. 2004 Jan; 115(1):19-30.
  • Guo, S.; Shu, D.; Simon, M.N.; Guo, P. Gene cloning, purification, and stoichiometry quantification of phi29 anti-receptor gp12 with potential use as special ligand for gene delivery Gene. 2003 Oct; 315 145-152.
  • Shu, D.; Huang, L.P.; Hoeprich, S.; Guo, P. Construction of phi29 DNA-packaging RNA monomers, dimers, and trimers with variable sizes and shapes as potential parts for nanodevices J Nanosci Nanotechnol. 2003 Aug; 3(4):295-302.
  • Hoeprich, S.; Zhou, Q.; Guo, S.; Shu, D. et al. Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus Gene Ther. 2003 Aug; 10(15):1258-1267.
  • Shu, D.; Guo, P. Only one pRNA hexamer but multiple copies of the DNA-packaging protein gp16 are needed for the motor to package bacterial virus phi29 genomic DNA Virology. 2003 Apr; 309(1):108-113.
  • Shu, D.; Guo, P. A viral RNA that binds ATP and contains a motif similar to an ATP-binding aptamer from SELEX J Biol Chem. 2003 Feb; 278(9):7119-7125.
  • Qin, J.; Tao, D.; Shu, D.; Leng, Y. et al. Cell cycle analysis by cyclin E + A/DNA multiparameter flow cytometry in exponential growth MOLT-4 cells Chin Med J (Engl). 2001 Sep; 114(9):967-971.
  • Shu, D.; Tao, D.; Gong, J. Transvalution of Sub-G1 and TDT method on apoptosis analyses Chin. J. Exp. Surg. 2000 Jan; 17 59-59.
  • Hu, X.; Zhu, B.; Hu, B.; Lin, H. et al. [An investigation of small marker chromosome in eight Turner syndrome patients by fluorescence in situ hybridization and DNA analysis] Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Dec; 16(6):392-394.
  • Shu, D.; Huang, W. Detection on immunization function in 20 cases Down's syndrome J.Chin. Engennics & Heredity. 1999 Jan; 7(3):116-116.
  • Gong, J.; Wei, W.; Shu, D. Cell proliferation Analysis by Cyclins/DNA Multiparameter Flow Cytometry Acta Univ. Med. Tongji. 1999 Jan; 28 177-177.
  • Tao, D.; Shu, D.; Gong, J. DNA content analysis and DNA strand breaks labelling method for detecting neototicoma apoptotic cells Chin. J. Med. Lab. Sci. 1999 Jan; 22 344-344.
  • Fang, J.; Ning, C.; Shu, D.; Wei, H. et al. Growth-promoting effect of recombinant human growth hormone and stanozolol in girls with Turner syndrome J Tongji Med Univ. 1999 Jan; 19(1):63-65.
  • Lin, H.; Hu, X.; Shu, D. Screening for fragile X syndrome in the children with mental retardation in Wuhan Acta Univ. Med. Tongji. 1999 Jan; 28(5):.
  • Shu, D.; Hu, X.; Lin, H. Cytogenetic study on abnormal X chromosome in 64 cases Chinese Journal of Medical Genetics. 1998 Jan; 15(4):259-259.
  • Xiong, Z.; Ning, C.; Shu, D. Analysis of 16 cases genetics metabolism disease in infant Chin. J. Pediatrics. 1998 Jan; 36(7):432-432.
  • Lin, H.; Hu, X.; Shu, D. Karyotype/phenotype correlation in females with somatic features of Turner's syndrome Acta Univ. Med. Tongji. 1996 Jan; 25(6):458-458.
  • Hu, X.; Zhu, B.; Lin, H.; Shu, D. et al. Study on the relationship between cytogenetics and phenotypic effect in Turner's syndrome J Tongji Med Univ. 1996 Jan; 16(4):245-248.
  • Hu, X.; Lin, H.; Shu, D. The phenotypic effects of X inactivation in Turner's syndrome Acta Univ. Med. Tongji. 1996 Jan; 25(2):144-144.
  • Hu, X.; Lin, H.; Shu, D. Studies in DNA replication patterns in human structural abnormality of X chromosome Chin. J. Engennics & Heredity. 1995 Jan; 3(4):72-72.
  • Ning, C.; Wei, H.; Shu, D.; Hu, X.F. et al. Clinical significance of multiple hypothalamic-pituitary functions assessment in patients with Turner's syndrome J Tongji Med Univ. 1994 Jan; 14(4):220-223.
  • Shu, D.; Hu, X.; Ning, C. Detection of Erythrocyte and serum Zine and Copper on hyperthyroidism J. Wuhan Med. 1994 Jan; 18(1):55-55.
  • Ning, C.; Wei, H.; Shu, D.; Hu, X. et al. Clinical significant of Hypothalamus-Pituitarium axis function detection in Turner's syndrome Chin. J. Pediatrics. 1994 Jan; 33(3):165-165.
  • Ning, C.; Liu, S.R.; Wei, H.; Wang, S. et al. The screening diagnosis of tetrahydrobiopterin deficient phenylketonuria J Tongji Med Univ. 1992 Jan; 12(4):216-218.
  • Hu, X.; Tao, D.; Shu, D. Determination of three enzyme activities of sphingolipidoses in tears Chin. J. Med. Genetics. 1992 Jan; 9(2):103-103.
  • Ning, C.; Wei, H.; Shu, D. Measurement of Hypothalamus-Pituitarium axis function with complex stimulation test J. Practical Pediatrics. 1992 Jan; 7(4):185-185.
  • Hu, X.; Tao, D.; Shu, D. Chromosome balanced translocation resulting in early spontaneous abortions Chin. J. Med. Genetics. 1992 Jan; 9(3):189-189.
  • Hu, X.; Shu, D. Cytogenetic study on chromosome in 735 cases J. Wuhan Med. 1991 Jan; 15(4):233-233.
  • Shu Y, Yin H, Rajabi M, Li H, Vieweger M, Guo S, Shu D, Guo P. RNA-based micelles: A novel platform for paclitaxel loading and delivery. J Control Release. 2018 Apr 28;276:17-29.
  • Smith JA, Braga A, Verheyen J, Basilico S, Bandiera S, Alfaro-Cervello C, Peruzzotti-Jametti L, Shu D, Haque F, Guo P, Pluchino S. RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity. Mol Ther Nucleic Acids. 2018 Mar 02;10:103-121.
  • Xu C, Haque F, Jasinski DL, Binzel DW, Shu D, Guo P. Favorable biodistribution, specific targeting and conditional endosomal escape of RNA nanoparticles in cancer therapy. Cancer Lett. 2018 02 01;414:57-70.
  • Yang X, Xu Y, Wang T, Shu D, Guo P, Miskimins K, Qian SY. Erratum to "Inhibition of cancer migration and invasion by knocking down delta-5-desaturase in COX-2 overexpressed cancer cells" [Redox Biol. 11 (2017) 653-662]. Redox Biol. 2017 08;12:1062.
  • Lee TJ, Yoo JY, Shu D, Li H, Zhang J, Yu JG, Jaime-Ramirez AC, Acunzo M, Romano G, Cui R, Sun HL, Luo Z, Old M, Kaur B, Guo P, Croce CM. RNA Nanoparticle-Based Targeted Therapy for Glioblastoma through Inhibition of Oncogenic miR-21. Mol Ther. 2017 07 05;25(7):1544-1555.
  • Yang X, Xu Y, Wang T, Shu D, Guo P, Miskimins K, Qian SY. Inhibition of cancer migration and invasion by knocking down delta-5-desaturase in COX-2 overexpressed cancer cells. Redox Biol. 2017 04;11:653-662.
  • Zhang Y, Leonard M, Shu Y, Yang Y, Shu D, Guo P, Zhang X. Overcoming Tamoxifen Resistance of Human Breast Cancer by Targeted Gene Silencing Using Multifunctional pRNA Nanoparticles. ACS Nano. 2017 01 24;11(1):335-346.
  • Zhao Z, Zhang H, Shu D, Montemagno C, Ding B, Li J, Guo P. Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination. Small. 2017 Jan;13(1).
  • Li H, Zhang K, Pi F, Guo S, Shlyakhtenko L, Chiu W, Shu D, Guo P. Controllable Self-Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting. Adv Mater. 2016 Sep;28(34):7501-7.
  • Binzel DW, Shu Y, Li H, Sun M, Zhang Q, Shu D, Guo B, Guo P. Specific Delivery of MiRNA for High Efficient Inhibition of Prostate Cancer by RNA Nanotechnology. Mol Ther. 2016 08;24(7):1267-77.