Dan Shu, MD

Research Associate Professor

Pharmaceutics and Pharmacology

Research Areas: The Division of Pharmaceutics & Pharmacology, Nanotechnology & Drug Delivery Systems, Cancer

Biography

Dr. Shu’s research is focused on (1) RNA nanotechnology: use RNA as a nanotechnology platform to construct RNA nanoparticles with functional modalities for therapeutics, targeting, and diagnostics (ACS Nano, 2015; Nature Nanotechnology, 2011; Nano Letters, 2004); and (2) Single molecule biophysical studies: use single-molecule detection (such as FRET) to understand the structure-function relationship for individual biomolecules (ACS Nano, 2010; EMBO Journal, 2007; RNA, 2007). Her long-term objective is to focus on the development and translation of RNA nanotechnology-based therapeutics and imaging agents into clinical trials.

Education

1990 - M.D., Tongji Medical University

Publications

Proof-of-concept for speedy development of rapid and simple at-home method for potential diagnosis of early COVID-19 mutant infections using nanogold and aptamer, Nanomedicine: Nanotechnology, Biology and Medicine. 2022 Sep | journal-article. doi: 10.1016/j.nano.2022.102590.
Rational design for controlled release of Dicer-substrate siRNA harbored in phi29 pRNA-based nanoparticles, Molecular Therapy - Nucleic Acids. 2021 Sep | journal-article. doi: 10.1016/j.omtn.2021.07.021.
Stoichiometry of multi-specific immune checkpoint RNA Abs for T cell activation and tumor inhibition using ultra-stable RNA nanoparticles, Molecular Therapy - Nucleic Acids. 2021 Jun | journal-article. doi: 10.1016/j.omtn.2021.03.007.
EpCAM-Targeted 3WJ RNA Nanoparticle Harboring Delta-5-Desaturase siRNA Inhibited Lung Tumor Formation via DGLA Peroxidation, Molecular Therapy - Nucleic Acids. 2020 Dec | journal-article. doi: 10.1016/j.omtn.2020.08.024.
Growth inhibitory and anti-metastatic activity of epithelial cell adhesion molecule targeted three-way junctional delta-5-desaturase siRNA nanoparticle for breast cancer therapy, Nanomedicine: Nanotechnology, Biology and Medicine. 2020 Nov | journal-article. doi: 10.1016/j.nano.2020.102298.
Specific Delivery of SiRNA to Tumor Cells via Innovative pRNA Nanoparticles: A Novel Promising Cancer Treatment Strategy, Soft Matter and Biomaterials on the Nanoscale. 2020 Aug | book-chapter. doi: 10.1142/9789811218026_0008.
Delivery of Anti-miRNA for Triple-Negative Breast Cancer Therapy Using RNA Nanoparticles Targeting Stem Cell Marker CD133, Molecular Therapy. 2019 Jul | journal-article. doi: 10.1016/j.ymthe.2019.04.018.
Specific delivery of delta-5-desaturase siRNA via RNA nanoparticles supplemented with dihomo-γ-linolenic acid for colon cancer suppression, Redox Biology. 2019 Feb | journal-article. doi: 10.1016/j.redox.2018.101085.
RNA Micelles for the Systemic Delivery of Anti-miRNA for Cancer Targeting and Inhibition without Ligand, ACS Nano. 2019 Jan | journal-article. doi: 10.1021/acsnano.8b07948.
RNA-based micelles: A novel platform for paclitaxel loading and delivery, Journal of Controlled Release. 2018 Apr | journal-article. doi: 10.1016/j.jconrel.2018.02.014.
RNA Nanotherapeutics for the Amelioration of Astroglial Reactivity, Molecular Therapy - Nucleic Acids. 2018 Mar | journal-article. doi: 10.1016/j.omtn.2017.11.008.
Favorable biodistribution, specific targeting and conditional endosomal escape of RNA nanoparticles in cancer therapy, Cancer Letters. 2018 Feb | journal-article. doi: 10.1016/j.canlet.2017.09.043.
RNA Nanoparticle-Based Targeted Therapy for Glioblastoma through Inhibition of Oncogenic miR-21, Molecular Therapy. 2017 Jul | journal-article. doi: 10.1016/j.ymthe.2016.11.016.
Inhibition of cancer migration and invasion by knocking down delta-5-desaturase in COX-2 overexpressed cancer cells, Redox Biology. 2017 Apr | journal-article. doi: 10.1016/j.redox.2017.01.016.
Overcoming Tamoxifen Resistance of Human Breast Cancer by Targeted Gene Silencing Using Multifunctional pRNA Nanoparticles, ACS Nano. 2017 Jan | journal-article. doi: 10.1021/acsnano.6b05910.
Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination, Small. 2017 Jan | journal-article. doi: 10.1002/smll.201601600.
Controllable Self‐Assembly of RNA Tetrahedrons with Precise Shape and Size for Cancer Targeting, Advanced Materials. 2016 Sep | journal-article. doi: 10.1002/adma.201601976.
Specific Delivery of MiRNA for High Efficient Inhibition of Prostate Cancer by RNA Nanotechnology, Molecular Therapy. 2016 Jul | journal-article. doi: 10.1038/mt.2016.85.
Systemic Delivery of Anti-miRNA for Suppression of Triple Negative Breast Cancer Utilizing RNA Nanotechnology, ACS Nano. 2015 Oct | journal-article. doi: 10.1021/acsnano.5b02471.
Regression of Gastric Cancer by Systemic Injection of RNA Nanoparticles Carrying both Ligand and siRNA, Scientific Reports. 2015 Sep | journal-article. doi: 10.1038/srep10726.
RNA Nanoparticles Derived from Three-Way Junction of Phi29 Motor pRNA Are Resistant to I-125 and Cs-131 Radiation, Nucleic Acid Therapeutics. 2015 Aug | journal-article. doi: 10.1089/nat.2014.0525.
New approach to develop ultra-high inhibitory drug using the power function of the stoichiometry of the targeted nanomachine or biocomplex, Nanomedicine. 2015 Jul | journal-article. doi: 10.2217/nnm.15.37.
RNA nanoparticle as a vector for targeted siRNA delivery into glioblastoma mouse model, Oncotarget. 2015 Jun | journal-article. doi: 10.18632/oncotarget.3632.
Ocular Delivery of pRNA Nanoparticles: Distribution and Clearance After Subconjunctival Injection, Pharmaceutical Research. 2014 Apr | journal-article. doi: 10.1007/s11095-013-1226-x.
Stable RNA nanoparticles as potential new generation drugs for cancer therapy, Advanced Drug Delivery Reviews. 2014 Feb | journal-article. doi: 10.1016/j.addr.2013.11.006.
Programmable folding of fusion RNA in vivo and in vitro driven by pRNA 3WJ motif of phi29 DNA packaging motor, Nucleic Acids Research. 2014 Jan | journal-article. doi: 10.1093/nar/gkt885.
Fabrication of pRNA nanoparticles to deliver therapeutic RNAs and bioactive compounds into tumor cells, Nature Protocols. 2013 Sep | journal-article. doi: 10.1038/nprot.2013.097.
Fabrication of 14 different RNA nanoparticles for specific tumor targeting without accumulation in normal organs, RNA. 2013 Jun | journal-article. doi: 10.1261/rna.037002.112.
Ultrastable synergistic tetravalent RNA nanoparticles for targeting to cancers, Nano Today. 2012 Aug | journal-article. doi: 10.1016/j.nantod.2012.06.010.
Thermodynamically stable RNA three-way junction for constructing multifunctional nanoparticles for delivery of therapeutics, Nature Nanotechnology. 2011 Oct | journal-article. doi: 10.1038/nnano.2011.105.
Assembly of multifunctional phi29 pRNA nanoparticles for specific delivery of siRNA and other therapeutics to targeted cells, Methods. 2011 Jun | journal-article. doi: 10.1016/j.ymeth.2011.01.008.
Dual-Channel Single-Molecule Fluorescence Resonance Energy Transfer to Establish Distance Parameters for RNA Nanoparticles, ACS Nano. 2010 Nov | journal-article. doi: 10.1021/nn1014853.
One-Way Traffic of a Viral Motor Channel for Double-Stranded DNA Translocation, Nano Letters. 2010 Sep | journal-article. doi: 10.1021/nl101939e.
Design and application of single fluorophore dual-view imaging system containing both the objective- and prism-type TIRF, Single Molecule Spectroscopy and Imaging III. 2010 Feb | journal-article. doi: 10.1117/12.847457.
Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motor, Biomedical Microdevices. 2010 Feb | journal-article. doi: 10.1007/s10544-009-9364-y.
Construction of Bacteriophage Phi29 DNA Packaging Motor and its Applications in Nanotechnology and Therapy, Annals of Biomedical Engineering. 2009 Oct | journal-article. doi: 10.1007/s10439-009-9723-0.
Approaches for stoichiometry and distance determination of nanometer bio-complex by dual-channel single molecule imaging, 2009 IEEE/NIH Life Science Systems and Applications Workshop. 2009 Apr | journal-article. doi: 10.1109/lissa.2009.4906725.
Fabrication of polyvalent therapeutic RNA nanoparticles for specific delivery of siRNA, ribozyme and drugs to targeted cells for cancer therapy, 2009 IEEE/NIH Life Science Systems and Applications Workshop. 2009 Apr | journal-article. doi: 10.1109/lissa.2009.4906696.
Evaluation of specific delivery of chimeric phi29 pRNA/siRNA nanoparticles to multiple tumor cells, Molecular BioSystems. 2009 Jan | journal-article. doi: 10.1039/b903428e.
Reversible Switching of pRNA Activity on the DNA Packaging Motor of Bacteriophage phi29, Journal of the American Chemical Society. 2008 Dec | journal-article. doi: 10.1021/ja806075d.
Bright-field analysis of phi29 DNA packaging motor using a magnetomechanical system, Applied Physics Letters. 2008 Oct | journal-article. doi: 10.1063/1.3000606.
Modular assembly of chimeric phi29 packaging RNAs that support DNA packaging, Biochemical and Biophysical Research Communications. 2008 Aug | journal-article. doi: 10.1016/j.bbrc.2008.05.094.
Instrumentation and metrology for single RNA counting in biological complexes or nanoparticles by a single-molecule dual-view system, RNA. 2007 Oct | journal-article. doi: 10.1261/rna.587607.
Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system, The EMBO Journal. 2007 Jan | journal-article. doi: 10.1038/sj.emboj.7601506.
Bottom-up Assembly of RNA Arrays and Superstructures as Potential Parts in Nanotechnology, Nano Letters. 2004 Sep | journal-article. doi: 10.1021/nl0494497.
A simple mathematical formula for stoichiometry quantification of viral and nanobiological assemblage using slopes of log/log plot curves, Journal of Virological Methods. 2004 Jan | journal-article. doi: 10.1016/j.jviromet.2003.08.015.
Gene cloning, purification, and stoichiometry quantification of phi29 anti-receptor gp12 with potential use as special ligand for gene delivery, Gene. 2003 Oct | journal-article. doi: 10.1016/s0378-1119(03)00729-7.
Bacterial virus phi29 pRNA as a hammerhead ribozyme escort to destroy hepatitis B virus, Gene Therapy. 2003 Aug | journal-article. doi: 10.1038/sj.gt.3302002.
Construction of phi29 DNA-Packaging RNA Monomers, Dimers, and Trimers with Variable Sizes and Shapes as Potential Parts for Nanodevices, Journal of Nanoscience and Nanotechnology. 2003 Aug | journal-article. doi: 10.1166/jnn.2003.160.
Only one pRNA hexamer but multiple copies of the DNA-packaging protein gp16 are needed for the motor to package bacterial virus phi29 genomic DNA, Virology. 2003 Apr | journal-article. doi: 10.1016/s0042-6822(03)00011-4.
A Viral RNA That Binds ATP and Contains a Motif Similar to an ATP-binding Aptamer from SELEX, Journal of Biological Chemistry. 2003 Feb | journal-article. doi: 10.1074/jbc.m209895200.
Growth-promoting effect of recombinant human growth hormone and stanozolol in girls with Turner syndrome, Journal of Tongji Medical University. 1999 Mar | journal-article. doi: 10.1007/bf02895600.
Study on the relationship between cytogenetics and phenotypic effect in Turner’s syndrome, Journal of Tongji Medical University. 1996 Dec | journal-article. doi: 10.1007/bf02888117.
Clinical significance of multiple hypothalamic-pituitary functions assessment in patients with Turner’s syndrome, Journal of Tongji Medical University. 1994 Dec | journal-article. doi: 10.1007/bf02897672.
The screening diagnosis of tetrahydrobiopterin deficient phenylketonuria, Journal of Tongji Medical University. 1992 Dec | journal-article. doi: 10.1007/bf02887852.