Emanuele Cocucci, MD, PhD

Assistant Professor

Pharmaceutics and Pharmacology

Research Areas: Chemical Biology, Cancer, Nanotechnology & Drug Delivery Systems, The Division of Pharmaceutics & Pharmacology

cocucci.1@osu.edu

(614) 366-4412

Professional Interests

Dr. Cocucci studies basic mechanisms of membrane trafficking and is interested in how these processes deviate during cancer development when compared to normal cells. His research adopts multiple techniques, including traditional biochemistry, cell biology, and high resolution fluorescent live cell microscopy. Dr. Cocucci’s goal is to define novel targets for cancer therapy and to improve drug delivery, studying the internalization pathways and the mechanisms of endosomal escape adopted by artificial and biological nanovectors.

Education

2008, PhD - Molecular Medicine, Vita-Salute San Raffaele University
2004, Board in Pharmacology - Clinical Pharmacology, Universita degli Studi di Milano
2000, MD - Medicine and Surgery, Universita degli Studi di Milano

Honors

Advanced Imaging Center (AIC), 2017
GlaxoSmithKline and Immune Disease Institute Fellowship, 2008 - 2010
FEBS Short Term Fellowship, 2006
Marie Curie Fellowship, 2002 - 2003

Publications

Data from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.c.6545634.v1.
Data from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.c.6545634.
Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.22528158.v1.
Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.22528158.
Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.22528161.v1.
Supplementary Figure from Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, . 2023 Apr | preprint. doi: 10.1158/1541-7786.22528161.
Redefining CD56 as a Biomarker and Therapeutic Target in Multiple Myeloma, Molecular Cancer Research. 2022 Jul | journal-article. doi: 10.1158/1541-7786.MCR-21-0828.
Extracellular Vesicle exchange is favored by cell proximity, . 2022 May | preprint. doi: 10.1101/2022.05.10.491399.
De novo endocytic clathrin coats develop curvature at early stages of their formation., Developmental cell. 2021 Nov | journal-article. doi: 10.1016/j.devcel.2021.10.019.
Optimizing extracellular vesicles' isolation from chronic lymphocytic leukemia patient plasma and cell line supernatant., JCI insight. 2021 Aug | journal-article. doi: 10.1172/jci.insight.137937.
CALM supports clathrin-coated vesicle completion upon membrane tension increase, Proceedings of the National Academy of Sciences. 2021 Jun | journal-article. doi: 10.1073/pnas.2010438118.
Microscopy approaches to study extracellular vesicles., Biochimica et biophysica acta. General subjects. 2020 Sep | journal-article. doi: 10.1016/j.bbagen.2020.129752.
Imaging intercellular interaction and extracellular vesicle exchange in a co-culture model of chronic lymphocytic leukemia and stromal cells by lattice light-sheet fluorescence microscopy., Methods in enzymology. 2020 Aug | journal-article. doi: 10.1016/bs.mie.2020.06.015.
Live imaging of intra-lysosome pH in cell lines and primary neuronal culture using a novel genetically encoded biosensor., Autophagy. 2020 Jun | journal-article. doi: 10.1080/15548627.2020.1771858.
Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop, Journal of Extracellular Vesicles. 2019 Dec | journal-article. doi: 10.1080/20013078.2019.1684862.
Endocytic Clathrin Coats Develop Curvature at Early Stages of Their Formation, . 2019 Jul | other. doi: 10.1101/715219.
Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines., Journal of extracellular vesicles. 2018 Nov | journal-article. doi: 10.1080/20013078.2018.1535750.
Measuring Clathrin-Coated Vesicle Formation with Single-Molecule Resolution., Methods in molecular biology (Clifton, N.J.). 2018 Jan | journal-article. doi: 10.1007/978-1-4939-8719-1_15.
Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy., Methods in molecular biology (Clifton, N.J.). 2017 Jan | journal-article. doi: 10.1007/978-1-4939-7253-1_19.
MYC mediates large oncosome-induced fibroblast reprogramming in prostate cancer, Cancer Research. 2017 Jan | journal-article. doi: 10.1158/0008-5472.CAN-16-2942.
Role of Passive Diffusion, Transporters, and Membrane Trafficking-Mediated Processes in Cellular Drug Transport, Clinical Pharmacology and Therapeutics. 2017 Jan | journal-article. doi: 10.1002/cpt.545.
Membrane dynamics of dividing cells imaged by lattice light-sheet microscopy, Molecular Biology of the Cell. 2016 Jan | journal-article. doi: 10.1091/mbc.E16-03-0164.
EVpedia: A community web portal for extracellular vesicles research, Bioinformatics. 2015 Jan | journal-article. doi: 10.1093/bioinformatics/btu741.
Ectosomes and exosomes: Shedding the confusion between extracellular vesicles, Trends in Cell Biology. 2015 Jan | journal-article. doi: 10.1016/j.tcb.2015.01.004.
Meeting report: Discussions and preliminary findings on extracellular RNA measurement methods from laboratories in the NIH Extracellular RNA Communication Consortium, Journal of Extracellular Vesicles. 2015 Jan | journal-article. doi: 10.3402/jev.v4.26533.
Dynamin recruitment and membrane scission at the neck of a clathrin-coated pit, Molecular Biology of the Cell. 2014 Jan | journal-article. doi: 10.1091/mbc.E14-07-1240.
Single-molecule analysis of fluorescently labeled G-protein-coupled receptors reveals complexes with distinct dynamics and organization, Proceedings of the National Academy of Sciences of the United States of America. 2013 Jan | journal-article. doi: 10.1073/pnas.1205798110.
Dynamics of Intracellular Clathrin/AP1- and Clathrin/AP3-Containing Carriers, Cell Reports. 2012 Jan | journal-article. doi: 10.1016/j.celrep.2012.09.025.
Errarum: Ectosomes (Current Biology (2011) 21 (R940-R941)), Current Biology. 2012 Jan | journal-article. doi: 10.1016/j.cub.2012.07.014.
Limited transferrin receptor clustering allows rapid diffusion of canine parvovirus into clathrin endocytic structures, Journal of Virology. 2012 Jan | journal-article. doi: 10.1128/JVI.07194-11.
The first five seconds in the life of a clathrin-coated pit, Cell. 2012 Jan | journal-article. doi: 10.1016/j.cell.2012.05.047.
Vesiclepedia: A Compendium for Extracellular Vesicles with Continuous Community Annotation, PLoS Biology. 2012 Jan | journal-article. doi: 10.1371/journal.pbio.1001450.
ARFGAP1 promotes AP-2-dependent endocytosis, Nature Cell Biology. 2011 Jan | journal-article. doi: 10.1038/ncb2221.
Ectosomes, Current Biology. 2011 Jan | journal-article. doi: 10.1016/j.cub.2011.10.011.
Distinct dynamics of endocytic clathrin-coated pits and coated plaques, PLoS Biology. 2009 Jan | journal-article. doi: 10.1371/journal.pbio.1000191.
Shedding microvesicles: artefacts no more, Trends in Cell Biology. 2009 Jan | journal-article. doi: 10.1016/j.tcb.2008.11.003.
The regulated exocytosis of enlargeosomes is mediated by a SNARE machinery that includes VAMP4, Journal of Cell Science. 2008 Jan | journal-article. doi: 10.1242/jcs.032029.
Enlargeosome traffic: Exocytosis triggered by various signals is followed by endocytosis, membrane shedding or both, Traffic. 2007 Jan | journal-article. doi: 10.1111/j.1600-0854.2007.00566.x.
The Ca2+-dependent exocytosis of enlargeosomes is greatly reinforced by genistein via a non-tyrosine kinase-dependent mechanism, FEBS Letters. 2007 Jan | journal-article. doi: 10.1016/j.febslet.2007.09.026.
Macropinocytosis: Regulated coordination of endocytic and exocytic membrane traffic events, Journal of Cell Science. 2006 Jan | journal-article. doi: 10.1242/jcs.03238.
Non-secretory exocytoses in the brain, Journal of Physiology Paris. 2006 Jan | journal-article. doi: 10.1016/j.jphysparis.2005.12.081.
Enlargeosome, an exocytic vesicle resistant to nonionic detergents, undergoes endocytosis via a nonacidic route, Molecular Biology of the Cell. 2004 Jan | journal-article. doi: 10.1091/mbc.E04-07-0577.
Regulated exocytosis: A novel, widely expressed system, Nature Cell Biology. 2002 Jan | journal-article. doi: 10.1038/ncb888.